產(chǎn)品介紹

BD 固定/透化試劑盒554714，

BD Cytofix/Cytoperm™ Fixation/Permeabilization Kit

本試劑盒用于細(xì)胞固定和破膜，以便使用熒光標(biāo)記的抗細(xì)胞因子抗體對(duì)胞內(nèi)細(xì)胞因子進(jìn)行染色。

試劑盒組成

1. 固定/破膜液（Fixation/Permeabilization Solution）

2. BD Perm/Wash™ 緩沖液

操作流程

1. 細(xì)胞固定與破膜：使用固定/破膜液處理細(xì)胞。

2. 洗滌與抗體稀釋：采用 BD Perm/Wash™ 緩沖液 清洗細(xì)胞，并稀釋抗細(xì)胞因子抗體用于染色。

This kit enables the fixation and permeabilization of cells which is necessary for staining intracellular cytokines with fluorochrome-conjugated anti-cytokine antibodies. The kit provides two reagents, fixation/permeabilization solution and BD Perm/Wash™ Buffer. After cell fixation and permeabilization, the BD Perm/Wash™ Buffer is used to wash the cells and to dilute the anti-cytokine antibodies for staining.

BD 固定/透化試劑盒554714，BD Cytofix/Cytoperm™ Fixation/Permeabilization Kit